Synthesis and Evaluation of 5-Chloro-2-Methoxy-N-(4-Sulphamoylphenyl) Benzamide Derivatives as Anti-cancer Agents

H NMR signals are reported with chemical shift values δ (ppm), multiplicity (s=singlet, d=doublet, t=triplet, q=quartet, m=multiplet and br=broad), relative integral, coupling constants J (Hz) and assignments.Mass spectra were recorded on an AB SCIEX TripleTOF ® 5600 mass spectrometer, and ionisation of all samples was carried out using ESI.Melting points were determined on an Electrothermal IA Abstract Sulphonamides embrace a sublime class of drugs with various biological activities.Since the discovery of E7010 in 1992, several sulphonamides as anti-cancer drug candidates have been identified.Herein, a new series of 5-chloro-2-methoxy-N-(4-sulphamoylphenyl)benzamide derivatives was synthesised, and their anti-proliferative activity was evaluated against human ovarian cancer (A2780) and colon cancer (HCT-116) cell lines.As one of the most potent antiproliferative agents, compound 4j was further tested against a panel of cancer cell lines revealing that human pancreatic carcinoma (MIA PaCa-2) displayed the highest sensitivity.Cellular mechanistic studies on MIA PaCa-2 cells showed that 4j arrested the G2/M cell cycle and induced apoptosis.GI 50 = 1.9 µM in MIA PaCa-2 pancreatic carcinoma