Supercritical CO 2 (SC‐CO 2 ) was used to extract natural food colors from Annatto ( Bixa orellana ) seeds with different pressures and temperatures. Results from the reverse thin‐layer chromatographic separation and GC/MS revealed that SC‐CO 2 extracts were composed mainly of bixin, geranylgeraniol and norbixin. Differences in absolute values of solubility parameters (SPD) between these main compounds and SC‐CO 2 indicated bixin was more easily extracted than norbixin under conditions used. A higher yield of total pigments was obtained at extraction pressure above 310 bar than at 210 bar and 50°C.
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Chao et al. (1991) studied this question.
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