Induction With Carbon Tetrachloride of Liver-Cell Carcinomas in Hamsters23

Carbon tetrachloride was administered by stomach tube to 10 female and 10 male Syrian golden hamsters, in weekly doses of 6.25 to 12.5 µl for 30 weeks. The livers of the animals dying during or after the period of treatment showed postnecrotic cirrhosis with regenerative hyperplastic nodules. All the animals, 5 females and 5 males, dying or killed 13 to 25 weeks after the end of the treatment, had 1 or more liver-cell carcinomas. No cholangiomatous tumors were observed. The induction, with N-2-fluorenylacetamide, of only cholangiomatous tumors and, with carbon tetrachloride, of only liver-cell tumors is discussed.