Normalization of Two-Dimensional Electrophoretic Patterns of Human Plasma Proteins and Comparisons of Cerebrospinal Fluid and Urine Patterns in Terms of the Normalized Map

A "normalized map" of human plasma proteins, which illustrated the standard distribution pattern of the proteins, was prepared by comparing eight two-dimensional electrophoretic gels of normal human plasma. Phenotypes of several plasma proteins were detected. The pI and molecular weight of each spot in the normalized map were calculated and 84 spots out of 128 were identified or tentatively identified as known plasma proteins by several methods including micro amino acid analysis of the proteins extracted from the stained gel pieces. Human cerebrospinal fluid and urine protein patterns were compared with the normalized map and the positions of proteins characteristic of each body fluid sample were determined.