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Methodological report reveals a novel technique for determining total urinary nitrogen in clinical samples, suggesting expanded feasibility beyond specialized hospital laboratories.
No one analytical method has done so much to further metabolism investigations as Kjeldahl’s method for the determination of total nitrogen. While applicable to all kinds of nitrogenous products of interest to the biochemist it has proved particularly serviceable in urine analysis. In its modern modifications it is one of the most rapid, convenient and accurate methods we have. At first sight it might therefore seem a thankless and superfluous task to attempt to find a substitute for such an admirable tool for research. Our original idea in attempting to find another method for the determination of total nitrogen in urine was, however, to fill a gap which Kjeldahl’s method does not fill; it just falls short of being suitable for clinical work except in the very best hospital laboratories.
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Folin et al. (1912) studied this question.
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