Prescreens of bacteria for biological control of soilborne diseases traditionally consist of tests for antibiosis in vitro. Two in vivo assays were developed as possible alternative prescreens for selecting candidate biological control agents for preemergence and postemergence damping-off diseases of cotton caused by Rhizoctonia solani. In the «radicle assay,» radicles from surface-disinfested seed were inoculated with R. solani. In the «hypocotyl assay,» excised surface-disinfested hypocotyls were inoculated with the pathogen (...)
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Joseph W. Kloepper (1991) studied this question.