Yearling lake trout (Salvelinus namaycush) were anesthetized with a 150 ppm solution of M.S. 222 for 2 min at 10 C. When tested in a vertical temperature gradient, their behavior was abnormal for 5 days following anesthetization. Initially, they remained at the bottom of the gradient tank, as much as 63% of the time, and when they did swim up into the gradient, their temperature selection was much less precise than that of control fish. The percentage of fish on the bottom declined daily, and on the 6th day their temperature distribution did not differ from that of controls.
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Goddard et al. (1974) studied this question.