Thiophene Derivatives from the Aerial Part of Pluchea indica

Chemical investigation on the aerial part of Pluchea indica resulted in the isolation of two new thiophene derivatives, 2-(4-hydroxy-3-methoxybut-1-yn-1-yl)-5-(penta-1,3-diyn-1-yl)thiophene (1) and 2-(4-O-β-glucopyranosyl-3-hydroxybut-1-yn-1-yl)-5-(penta-1,3-diyn-1-yl)thiophene (2) along with three known thiophene derivatives 2-(3,4-dihydroxybut-1-yn-1-yl)-5-(penta-1,3-diyn-1-yl)thiophene (3), 2-(3-acetoxy-4-hydroxybut-1-yn-1-yl)-5-(penta-1,3-diyn-1-yl) thiophene ( 4) and 2-(prop-1-yn-1-yl)-5-(6-acetoxy-5-hydroxyhexa-1,3-diyn-1-yl) thiophene (5).The structures of 1 and 2 were determined on the bases of extensive spectroscopic analysis, including 1D and 2D NMR data.Antimicrobial activities of compounds 1-5 were tested.