Unique Transcriptional Signatures Observed in Stem Cells from the Dental Pulp of Deciduous Teeth Produced on a Large Scale

Background and Objective: For sharing transcriptomic signatures with their origin, each population of mesenchymal stem/stromal cells (MSCs) exhibits unique properties.This study aimed to identify the transcriptomic signature of Human Immature Dental Pulp Stem Cells (hIDPSCs), a special type of MSC. Materials and Methods: To provide further evidence which may support the distinctive neuroprotective, neuroregenerative properties of hIDPSCs, it was performed the transcriptome analysis of these cells produced on a large-scale using RNA-Seq.Data were analyzed using bioinformatics tools to obtain the list of differentially expressed genes (DEGs).The DEGs identified in the hIDPSCs were subjected to functional enrichment analysis.Results: The data obtained were compared with the public data of RNA-Se from 136 samples from different donors of Adipocyte-Derived (AD-MSC), Bone Marrow (BM-MSC), Hepatocyte-Derived (HD-MSC), Menstrual Blood (MB-MSC), Umbilical Cord (UC-MSC) and Vertebral Tissue (vMSC) MSCs.These analyses showed that the hIDPSC shares at least 72% of transcripts with MSC from other sources.However, the cells have a unique transcriptional signature characterized by the differential expression of genes that promote axon growth and guidance.Conclusion: The unique transcriptional signature of the hIDPSCs provides evidence that the NestaCell changes has neuro regenerative and neuroprotective actions, justifying the therapeutic effects we observed in both preclinical and clinical studies for neurodegenerative disorders.