A quantitative study by reflection–absorption infrared spectroscopy (RAIRS) on multilayer systems of Langmuir–Blodgett (LB) poly(amino acid) films has been carried out. Thickness measurements of monolayer and multilayer systems have been made and the results obtained by two well‐known equations have been compared. Evolution of the α‐helix conformation as a function of temperature in the case of polybenzyl aspartate (PBA) and polybenzyl glutamate LB systems has also been studied by RAIRS. Finally, a Fourier transform infrared transmission study on PBA LB multilayers as a function of temperature has allowed us to draw some conclusions on the orientation of the CO bonds (amide I) with respect to the dipping direction.
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Auduc‐Boyer et al. (1995) studied this question.
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