Layered enzyme assays: The use of a layered sol-gel based material allows for spatial separation of coimmobilized enzyme and fluorescent aptamer reporter species. This provides a route for facile assaying of enzyme activity and inhibition in cases where the aptamer signals the presence of the enzyme substrate. This then results in accurate IC50 determinations.
No takes yet. Share an insight, caveat, or question.
Rupcich et al. (2006) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: