Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China

Figures

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  Figure 1. Phylogenetic tree of global EV-A90 based on entire VP1 sequence. Isolates from Xinjiang were marked with and the prototype of EV-A90 (BAN00-10399) was marked with . The phylogenetic tree was reconstructed using neighbor-joining method with the substitution model of maximum composite likelihood model.

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  Figure 2. Potential recombination analysis of the whole genome of the Xinjiang EV-A90 strains. Similarity analysis (a) and bootscaning analysis (b) were performed in a 200-nt sliding window. Each point indicated similarity between the Xinjiang strain and other EV-A strains in a 20-nt moving step. Kimura (2-parameter) model was used in the analysis.

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  Figure 3. Phylogenetic tree of genetic fragments for EV-A90 and other similar serotypes, including P1, P2, P3 and 3CD, were illustrated in figure (a–d). Isolates in this research were marked with and the prototype of EV-A90 (BAN00-10399) was marked with . The phylogenetic tree was reconstructed using maximum likelihood method, in which GTR + I + G model was used in P1 fragment and TN93 + I + G model was used in the rest fragment. We also put forward a hypothesis between the major parental type (yellow) and the minor parental type (blue) because of recombination.

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  Figure 4. The viral titers of five EV-A90 strains in different time were drawn in the coordinate system. The blue line represented the growth curve in 36 °C and the red one represented the growth curve in 39.5 °C.