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We report a novel method for the generation of GUVs (generate unilamellar vesicles) from spin-coated lipid films by means of localized heating. This technique enables GUV formation from both charged and neutral lipid species, as well as from a complex lipid mixture, in various ionic strength conditions. Encapsulation was possible during and after GUV formation.
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Billerit et al. (2012) studied this question.
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