A Schiff's base of pyridoxal 5′-phosphate formed by reaction with n-hexylamine was reduced on a dropping mercury electrode. The polarographic reduction was studied as a function of pH and solvent composition in NN-dimethylformamide–water mixtures. Uptake of two protons before the rate-limiting step was observed in all cases.
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Llor et al. (1978) studied this question.
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