We have developed procedures to solubilize the envelope UDP‐galactose:diacylglycerol galactosyltransferase activity and to assay this enzyme after solubilization with a zwitterionic non‐denaturing detergent (CHAPS) and fractionation. From solubilized envelope membranes isolated from intact spinach chloroplasts, we were able to prepare by chromatography on hydroxyapatite a fraction enriched (6–7‐fold) in this enzyme responsible for monogalactosyldiacylglycerol synthesis.
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Covès et al. (1986) studied this question.
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