Adeno-associated virus 9 vector-mediated cardiac-selective expression of human secretory leukocyte protease inhibitor attenuates myocardial ischemia/reperfusion injury

Figures

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  Figure 1. Determination of body weight of experimental animal after viral treatment in I/R injury (A), heart weight (B), heart weight-to-body weight ratio (C). Determination of serum toxicity biomarkers after adeno-associated viral particles injection including AST (D), ALT (E), BUN (F), and creatinine (G). Determination of serum myocardium injury biomarkers after AAV9 injection including CK-MB (H), cTnI (I), and myoglobin (J) (*p < 0.05 vs. control).

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  Figure 2. Determination of slpi gene (A) and protein expression level (B) in organs of I/R injury rats treated with viral particles (*p < 0.05 vs. AAV9).

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  Figure 3. Determination of infarct size (A), area at risk (B), and serum cardiac biomarkers including cTnI (C), CK-MB (D), and myoglobin (E) in I/R rats (*p < 0.05 vs. sham) (#p < 0.05 vs. AAV9).

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  Figure 4. Determination of inflammatory cytokines after treating with viral particles including serum TNF-α level (A), IL-1β level (B), IL-6 (C), and cardiac TNF-α level (D), IL-1β level (E), and IL-6 (F) on I/R injury rats (*p < 0.05 vs. sham) (#p < 0.05 vs. AAV9).

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  Figure 5. Myocardial histology parameters consists of inflammation (A), degeneration (B), necrosis (C), and hemorrhage (D) (*p < 0.05 vs. sham) (#p < 0.05 vs. AAV9).

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  Figure 6. Determination of oxidatively modified cardiac protein level including tissue PC content level (A), serum PC content level (B), tissue IMA level (C), and serum IMA level (D) (*p < 0.05 vs. sham) (#p < 0.05 vs. AAV9).

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  Figure 7. A schematic diagram of the major findings in this study. The cardiac selective overexpression of SLPI by using AAV9-SLPI. Overexpression SLPI could provide the cardioprotective effect associated with the attenuation of inflammation, intracellular ROS production related to reducing infarct size and cardiac biomarkers and improving cardiac function. This figure generated by BioRender with access URL https://app.Biorender.com/illustrations/62a4bc9045f21a4fdcc51874.