Demonstrates a reliable technique for extracting pure myosin isoforms from single rat muscle fibers for in vitro motility assays without altering function.
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May enable reliable myosin isoform studies in vitro; leaves open translation to human cardiac muscle.
As skeletal muscle fibres mostly express a single myosin isoform, they are a potential source of pure myosin isoforms. A technique is described that allows extraction and identification of pure myosin isoforms from single fibres, and testing of such myosins in an in vitro motility assay (IVMA). The results show that the extraction procedure does not alter myosin function and support the view that single fibres are reliable sources of purified myosin isoforms for IVMA.
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Canepari et al. (1999) studied this question.
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