This unit includes protocols for the laboratory maintenance of the obligate intracellular bacterium Rickettsia rickettsii , including propagation in mammalian cell cultures, as well as isolation, counting, and storage procedures. Regulations for working with R. rickettsii in biosafety level 3 containment are also discussed. Curr. Protoc. Microbiol . 11:3A.5.1‐3A.5.21.
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Ammerman et al. (2008) studied this question.
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