We have investigated the activities of T cell populations able to cooperate in the humoral immune response to TNP coupled to the carriers SRBC or KLH. We used Poisson analyses in which small numbers of carrier-primed spleen cells were added to cultures containing an excess of B cells and antigen. We have demonstrated that we could a) detect the activity of a single helper T cell; b) calculate its frequency in a given spleen cell suspension; and c) calculate its relative activity in stimulating the humoral immune response.
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Marrack et al. (1974) studied this question.