A fluorophore-based bio-barcode amplification assay that relies on in situ detection of fluorophore-labeled barcodes attached to polystyrene (PS) microparticles rather than to gold nanoparticles has been developed; the method involves significantly fewer experimental steps and, thus, reduces the time for the assay without losing its ultrahigh sensitivity. The fluorescence image shows PS particles, on which barcode DNA strands labeled with a fluorophore dye (Alexa 647) have been hybridized with the barcode DNA capture strands.
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Oh et al. (2005) studied this question.
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