October 7, 2025Molecular Therapy — Nucleic Acids3 citationsOpen Access

mRNA vaccine expressing enterovirus D68 virus-like particles induces potent neutralizing antibodies and protects against infection

YKYuta KunishimaThe University of Osaka KSKota SenpukuThe University of Osaka CKChikako Kataoka-NakamuraThe University of Osaka

Structured PICO

Does an mRNA vaccine expressing EV-D68 VLPs induce neutralizing antibodies and protect against infection in a mouse model?

Population

Mice (including neonatal mice for passive serum transfer model) and HEK293 cells

Intervention

mRNA vaccine expressing EV-D68 virus-like particles (VLPs) via co-administration of P1 mRNA-LNP and 3CD mRNA-LNP

Comparator

Non-vaccinated (PBS), mRNA vaccine for VP1, or inactivated whole virion (IWV) vaccine

Outcome

Induction of anti-EV-D68 IgG and neutralizing antibodies, and protection against EV-D68 infection (viral titers, paralysis, and survival)surrogate

An mRNA vaccine expressing EV-D68 VLPs elicits robust systemic and mucosal immunity, protecting mice against EV-D68 infection and paralysis, and offering cross-protection against other enteroviruses.

Abstract

T cell responses. In conclusion, our results suggest that this mRNA vaccine is a promising candidate for EV-D68 prevention.

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