Morpho-Functional Basis Of Endothelial Dysfunction In Diabetes Mellitus

Gozhenko A. I., Kuznetsova H. S., Kuznetsova K. S., Kuznetsova O. M., Byts T. M., Zukow W. Morpho-functional basis of endothelial dysfunction in diabetes mellitus. Journal of Education, Health and Sport. 2017;7(6):516-524. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.822050 http://ojs.ukw.edu.pl/index.php/johs/article/view/4578 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 1223 (26.01.2017). 1223 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author 2017; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 03.06.2017. Revised: 20.06.2017. Accepted: 30.06.2017. MORPHO-FUNCTIONAL BASIS OF ENDOTHELIAL DYSFUNCTION IN DIABETES MELLITUS A. I. Gozhenko, H. S. Kuznetsova, K. S. Kuznetsova, O. M. Kuznetsova, T. M. Byts, * W. Zukow State Enterprise "Ukrainian Research Institute of Transport Medicine, Ministry of Health of Ukraine", Odessa, Ukraine *Wydział Nauk o Ziemi, Uniwersytet Mikołaja Kopernika w Toruniu Abstract The diabetes mellitus (DM) inevitably progresses and leads to complications, among which the main place is occupied by micro- and macroangiopathies. The presence of endothelial damage in DM can be established even before macroscopically significant damage to the vessel. At the same time, there is no summary ED characteristic for diabetes. The aim of the study is to make a comprehensive evaluation of ED in DM -1 and DM-2 types. Materials and methods of research. 60 persons, including 53 DM type 1 and type 2, with a severe course (state of decompensation) participated in the present study. We used the method of estimating ED by the number of circulating desquamation endothelial cells (CECs) at the stages of decomposition with simultaneous determination of NO2- and NO3- metabolites of nitric oxide. Results and discussion. In patients with diabetes, the level of CECs increased in 3-5 times and ranged from 1800 to 11,200 cells / ml. The average amount of CECs in patients with diabetes was 3358.5 ± 366.3 cells / ml. Conclusions: Endothelium is involved in the pathological process at DM. This is evidenced by a significant increase in CECs in the blood plasma. The use of this method allows to detect ED before clinically considerable vascular impairment and reflects the severity of the course and duration of DM.