In the course of ou^ previous on immune bacteriolysis <Am ano at ul. , 1954a, b, c), it was necessary to use quantitative determination.For this purpose, it \\, as necessary to separate serum spheroplasts from intact bacteria.\\!e sacceeded in the selective Iysis of serum spheroplasts by the addition of a definite amount of sodium desoxycholate.Thus we could apply the principle of quantitative assay of Mayer, Croft and Gray (1948) on immune hemolysis to our bacteriolytic system.The nucleic acid formed from Iysed bacteria was estimated after deproteinization.
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Amano et al. (1958) studied this question.