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Four edible oils, linseed, borage and sunflower seed oils, and the liver oil of shortfin mako (Isurus oxyrinchus), have been fractionated by open column liquid chromatography, using partially argentated silica gel as stationary phase, looking for purify polyunsaturated fatty acids (PUFA). By this method, linoleic (LA, 18:2n6),α-linolenic (ALA, 18:3n3), γ-linolenic (GLA, 18:3n6), stearidonic (SDA, 18:4n3), eicosapentaenoic (EPA, 20:5n3) and docosahexaenoic (DHA, 22:6n3) fatty acid esters have been isolated from these oils with high purity percentages and yields.
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Guil‐Guerrero et al. (2003) studied this question.
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