What question did this study set out to answer?

To evaluate the feasibility of full-length gene analysis across eight drug resistance-related genes using ion semiconductor next-generation sequencing compared to conventional phenotypic drug susceptibility testing in multidrug-resistant tuberculosis.

April 3, 2018Annals of Laboratory MedicineOpen Access

Determining Genotypic Drug Resistance by Ion Semiconductor Sequencing With the Ion AmpliSeq™ TB Panel in Multidrug-ResistantMycobacterium tuberculosisIsolates

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Authors

JPJoonhong ParkSK Group (South Korea)SSSo–Youn ShinCatholic University of Pusan KKKyungjong Kim

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Implication

Comparative study demonstrates high concordance between semiconductor sequencing and phenotypic testing in drug-resistant tuberculosis, highlighting its utility for genetic resistance profiling.

Key Points

To evaluate the feasibility of full-length gene analysis across eight drug resistance-related genes using ion semiconductor next-generation sequencing compared to conventional phenotypic drug susceptibility testing in multidrug-resistant tuberculosis.
Extracted genomic DNA from N=30 multidrug-resistant Mycobacterium tuberculosis isolates with phenotypic drug susceptibility profiles for eight standard anti-tuberculosis drugs.
Performed targeted next-generation sequencing of inhA, katG, rpoB, pncA, rpsL, embB, eis, and gyrA using the Ion AmpliSeq TB panel on an Ion PI Chip v3.
Demonstrated good agreement between genotypic and phenotypic drug susceptibility testing for ethambutol (Kappa = 0.800), fluoroquinolones (Kappa = 0.783), streptomycin (Kappa = 0.769), and pyrazinamide (Kappa = 0.734).
Identified 17 novel amino acid variants across five target genes: six in katG, five in rpoB, three in pncA, two in embB, and one in inhA.

Cite This Study

Park et al. (2018) studied this question.

synapsesocial.com/papers/6a73a14599c817e23aa73378 https://doi.org/10.3343/alm.2018.38.4.316

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Implication

Comparative study demonstrates high concordance between semiconductor sequencing and phenotypic testing in drug-resistant tuberculosis, highlighting its utility for genetic resistance profiling.

Determining Genotypic Drug Resistance by Ion Semiconductor Sequencing With the Ion AmpliSeq™ TB Panel in Multidrug-ResistantMycobacterium tuberculosisIsolates

Discussion

Member takes

Implication

Key Points

Cite This Study

Determining Genotypic Drug Resistance by Ion Semiconductor Sequencing With the Ion AmpliSeq™ TB Panel in Multidrug-ResistantMycobacterium tuberculosisIsolates

Discussion

Member takes

Implication

Key Points

Cite This Study

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