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Experiments were conducted to develop techniques for synthesizing chimeras between plants of known genotype by utilizing in vitro techniques Chimeral calli composed of green and albino tobacco cells were obtained by initiating callus tissue from mixtures of albino and green cotyledons, hypocotyls, callus cultures and cell suspensions The most effective mixing of genotypes occurred when callus was derived from mixed filtered cell suspensions Upon shoot regeneration, chimeral calli yielded 1317 non-chimeral and four chimeral plants Chimeras may have arisen as a result of experimental procedures or possibly from spontaneous chromosomal abnormalities since leaves of some albino control plants occasionally produced small green islands of cells Explanations for the recovery of a high percentage of non-chimeral shoots are presented
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Marcotrigiano et al. (1984) studied this question.
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