The lethal effects of methotrexate upon human tumor cells in vitro depend not only upon the concentration of the drug around those cells, but also upon the length of time for which the drug and the cells remain in contact. Short-term exposure to even very high concentrations of methotrexate may leave a large proportion of tumor cells intact, while longer exposures to lower drug concentrations will leave few cells capable of repeated divisions.
No takes yet. Share an insight, caveat, or question.
Roger Berry (1968) studied this question.
Synapse has enriched one closely related paper. Consider it for comparative context: