Similar affinities of ADP and ATP for G‐actin at physiological salt concentrations

The equilibrium constant for the exchange of ATP and ADP at G-actin was determined by fluorimetric titration of G-actin-bound epsilon-ATP by ATP or ADP. The affinity of ATP for G-actin was found to be only about 3-fold higher than the affinity of ADP for G-actin at 37 degrees C, pH 7.5 and physiologically relevant salt concentrations (100 mmol K+/l, 0.8 mmol Mg2+/l, less than 0.01 mmol Ca2+/l).