Outer Membrane is Critical for Viability of Cell Wall-deficient Bacterial Cells, L-form

Daisuke Shiomi; Taku Oshima

doi:10.2142/biophys.63.27

January 1, 2023Seibutsu ButsuriOpen Access

Outer Membrane is Critical for Viability of Cell Wall-deficient Bacterial Cells, L-form

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Key Points

To elucidate the conversion mechanism and survival requirements of cell wall-deficient L-form bacteria under hyperosmotic conditions.
Inhibited bacterial cell wall synthesis under hyperosmotic growth conditions to stimulate L-form conversion.
Employed real-time live-cell imaging to directly visualize the transformation and proliferation dynamics of L-forms.
Hyperosmotic conditions prevent osmotic lysis when bacterial cell walls are disrupted, permitting transition into viable L-form states.
Real-time visualization indicates that the outer membrane provides indispensable structural integrity necessary for cell viability and proliferation in the absence of a peptidoglycan cell wall.

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Implication

Laboratory study reveals outer membrane dependence for cell survival in wall-deficient L-form bacteria, highlighting non-canonical mechanisms of bacterial persistence.

Abstract

バクテリアは細胞壁合成が阻害されると溶菌するが，高浸透圧条件下では一部の細胞が細胞壁が無くても生存可能なL-formと呼ばれる状態に変換し，増殖できる．本稿では，その変換機構を明らかにすべく，我々の研究チームが行ったL-formのリアルタイム可視化による成果を中心に紹介する．

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Cite This Study

Shiomi et al. (2023) studied this question.

synapsesocial.com/papers/6a73dd646c6745e79da8be5d https://doi.org/10.2142/biophys.63.27

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Outer Membrane is Critical for Viability of Cell Wall-deficient Bacterial Cells, L-form

Key Points

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Implication

Abstract

Discussion

Cite This Study

Outer Membrane is Critical for Viability of Cell Wall-deficient Bacterial Cells, L-form

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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