Synaptophysin and glutamate decarboxylase expression in the rat cerebellum structures in cholestasis

The aim of the paper was to estimate by immunohistochemistry the state of the rat cerebellum cortex synapses and of gamma-aminobutyric acid (GABA)ergic neurons in cholestasis. Experimental rats were anesthetizedwith ethylic ether and the common bile duct was ligated 3-5 mm below the confluence of the lobar ducts. Animals of the control group underwent sham operation. On the 2 nd , 5 th , 10 th , 20 th , 45 th , and 90 th day the animals of the control and cholestasis groups were decapitated and the samples of their cerebellum cortex were prepared for immunohistochemistry to examine the synaptophysin (Syn) and glutamate decarboxylase (GAD) expression.