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Previous analysis of mtDNA control region (CR) sequence variability for grey wolf population from Bosnia&Herzegovina has revealed its high genetic variability and absence of population structure. Due to limited informativeness of CR mtDNA, additional analyses of population genetic variability and structure were done, applying high polymorphic genetic markers. Microsatellites were applied in order to explore level of genetic variability and population genetic structure. Eighteen microsatellites have been amplified in a total sample of 20 grey wolves, from different localities across Bosnia&Herzegovina. Observed values of the basic genetic parameters indicated high level of genetic variability (Ho=0.66; He=0.76), similar to other populations belonging to the grey wolf Dinaric-Balkan population. STRUCTURE analysis showed differentiation into two genetic clusters. These results are important for future management plans and estimation of the conservation units.
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Šnjegota et al. (2016) studied this question.
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