Impact of exudative diathesis induced by selenium deficiency on LncRNAs and their roles in the oxidative reduction process in broiler chick veins

// Changyu Cao 1, * , Ruifeng Fan 1, * , Jinxin Zhao 1 , Xia Zhao 1 , Jie Yang 1 , Ziwei Zhang 1 , Shiwen Xu 1, 2 1 Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, P. R. China 2 Key Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, P. R. China * These authors contributed equally to this work Correspondence to: Ziwei Zhang, email: zhangziwe@sina.com Shiwen Xu, email: shiwenxu@neau.edu.cn Keywords: exudative diathesis, selenium deficiency, long noncoding RNA-seq, oxidative reduction process, broiler chick vein Received: September 30, 2016      Accepted: January 24, 2017      Published: February 01, 2017 ABSTRACT Selenium deficiency may induce exudative diathesis (ED) in broiler chick, and this damage is closely related to oxidative damage. Long noncoding RNA (LncRNA) can regulate the redox state in vivo . The aim of the present study was to clarify the LncRNA expression profile in broiler veins and filter and verify the LncRNAs related to oxidative damage of ED. This study established an ED model induced by selenium deficiency and presented the expression and characterization of LncRNAs in normal and ED samples. A total of 15412 LncRNAs (including 8052 novel LncRNAs) were generated in six cDNA libraries using the Illumina Hi-Seq 4000 platform. 635 distinct changes in LncRNAs (up-regulated fold change > 1.5, down-regulated fold change < 0.67 and differentially expressed LncRNAs) were filtered. Gene ontology enrichment on LncRNAs target genes showed that the oxidative reduction process was important. This study also defined and verified 19 target mRNAs of 23 LncRNAs related to the oxidative reduction process. The in vivo and vitro experiments also demonstrated these 23 LncRNAs can participate in the oxidative reduction process. This study presents LncRNAs expression profile in broiler chick veins for the first time and confirmed 23 LncRNAs involving in the vein oxidative damage in ED.