Changes and theirsignificances of IFN- γ , TNF- α and IL-2 level in mouse peripheral blood followingintraperitoneal injection with influenza viruses

：Objective To observechanges of IFN-γ, TNF-α and IL-2 levels in mouseperipheral blood following intraperitoneal injection with influenza viruses. Methods Micewere divided into 3 groups randomly and injected with human H3N2 influenza virus, avianH9N2 influenza virus and sterilized virus-free allantoic fluid, respectively. Sera indifferent groups were collected at several time points after virus injection, and thelevels of IFN-γ, TNF-α and IL-2 in peripheral bloodwere tested by sandwich ELISA. Statistical analysis was made using analysis of varianceand LSD-t test. Results The levels of IFN-γand IL-2 inperipheral blood in influenza viruses injection groups were significantly higher thanthose in negative control group, and no significant difference in TNF-α level was found between influenza viruses injection groups andnegative control group. Conclusion Intraperitoneal injection with human or avian influenzaviruses can promote the production of IFN-γand IL-2 inmouse peripheral blood, but it has little effect on the production of TNF-α.