Some characteristics of polyvinyl alcohol (PVA) degrading enzyme produced by Pseudomonas O-3 which utilizes PVA as the sole carbon source were examined. The enzyme contains 1 atom iron per molecule and its solution has absorption maxima at 280, 365 and 470nm. It produced hydrogen peroxide with the degradation of PVA. Equivalence was established between the amounts of oxygen consumed and hydrogen peroxide produced, indicating that this enzyme belongs to an oxidase but not to an oxygenase. In its substrate specificity, it oxidizes secondary alcohols such as 2-pentanol, 2-hexanol, 3-hexanol, 2-heptanol, 3-heptanol and 4-heptanol. Among PVA analogues, it does not oxidize low molecular weight analogues such as dimer and trimer but oxidizes analogues with molecular weight larger than 300.
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Tomoo Suzuki (1978) studied this question.