What question did this study set out to answer?

The aim is to investigate the use of targeted small extracellular vesicles for delivering the CRISPR/Cas9 system to knock out the PD-1 gene in T cells.

August 6, 2026Applied Microbiology and BiotechnologyOpen Access

T cell engineering by targeted small extracellular vesicles delivering CRISPR/Cas9 system for PD-1 knock-out

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Authors

MFMahboube Shahrabi FarahaniTarbiat Modares University MSMehdi ShamsaraNational Institute of Genetic Engineering and Biotechnology SMSeyed Mohammad MoazzeniTarbiat Modares University

Discussion

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Implication

Randomized trial demonstrates enhanced T cell function after PD-1 gene knockout using targeted small extracellular vesicles, suggesting improved cancer immunotherapy efficacy.

Key Points

The aim is to investigate the use of targeted small extracellular vesicles for delivering the CRISPR/Cas9 system to knock out the PD-1 gene in T cells.
Targeted small extracellular vesicles (s-EVs) were loaded with CRISPR/Cas9 plasmids via electroporation.
T cells were treated with these s-EVs, and gene disruption was assessed using flow cytometry and sequencing.
Cytotoxicity and cytokine release were evaluated through CFSE staining and ELISA analyses.
Higher uptake of targeted s-EVs by T cells compared to non-targeted s-EVs was confirmed.
The knockout of the PD-1 gene in T cells led to significantly enhanced cytotoxicity and cytokine release.
Targeted s-EVs successfully delivered the CRISPR/Cas9 system, simplifying immunotherapy applications.

Cite This Study

Farahani et al. (2026) studied this question.

synapsesocial.com/papers/6a743730764cddc9499d4637 https://doi.org/10.1007/s00253-026-13976-x

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August 6, 2026Applied Microbiology and BiotechnologyOpen Access

T cell engineering by targeted small extracellular vesicles delivering CRISPR/Cas9 system for PD-1 knock-out

View Full Paper

Ask AI

Bookmark

Authors

MFMahboube Shahrabi FarahaniTarbiat Modares University MSMehdi ShamsaraNational Institute of Genetic Engineering and Biotechnology SMSeyed Mohammad MoazzeniTarbiat Modares University

Discussion

Member takes

Implication

Randomized trial demonstrates enhanced T cell function after PD-1 gene knockout using targeted small extracellular vesicles, suggesting improved cancer immunotherapy efficacy.

Key Points

The aim is to investigate the use of targeted small extracellular vesicles for delivering the CRISPR/Cas9 system to knock out the PD-1 gene in T cells.
Targeted small extracellular vesicles (s-EVs) were loaded with CRISPR/Cas9 plasmids via electroporation.
T cells were treated with these s-EVs, and gene disruption was assessed using flow cytometry and sequencing.
Cytotoxicity and cytokine release were evaluated through CFSE staining and ELISA analyses.
Higher uptake of targeted s-EVs by T cells compared to non-targeted s-EVs was confirmed.
The knockout of the PD-1 gene in T cells led to significantly enhanced cytotoxicity and cytokine release.
Targeted s-EVs successfully delivered the CRISPR/Cas9 system, simplifying immunotherapy applications.

Cite This Study

Farahani et al. (2026) studied this question.

synapsesocial.com/papers/6a743730764cddc9499d4637 https://doi.org/10.1007/s00253-026-13976-x

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