A chemically modified synthetic CRISPR RNA (scrRNA) mediated efficient genome editing in human cells, showing enhanced activity compared with unmodified crRNA.
Genome editing with the clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9 nuclease system is a powerful technology for manipulating genomes, including introduction of gene disruptions or corrections. Here we develop a chemically modified, 29-nucleotide synthetic CRISPR RNA (scrRNA), which in combination with unmodified transactivating crRNA (tracrRNA) is shown to functionally replace the natural guide RNA in the CRISPR-Cas9 nuclease system and to mediate efficient genome editing in human cells. Incorporation of rational chemical modifications known to protect against nuclease digestion and stabilize RNA-RNA interactions in the tracrRNA hybridization region of CRISPR RNA (crRNA) yields a scrRNA with enhanced activity compared with the unmodified crRNA and comparable gene disruption activity to the previously published single guide RNA. Taken together, these findings provide a platform for therapeutic applications, especially for nervous system disease, using successive application of cell-permeable, synthetic CRISPR RNAs to activate and then silence Cas9 nuclease activity.
Rahdar et al. (Mon,) reported a other. Chemically modified synthetic CRISPR RNA (scrRNA) vs. Unmodified crRNA and single guide RNA was evaluated on Genome editing and gene disruption activity. A chemically modified synthetic CRISPR RNA (scrRNA) mediated efficient genome editing in human cells, showing enhanced activity compared with unmodified crRNA.