By varying the interval between two stimuli it is possible to measure the activation heat in a skeletal muscle twitch. The method depends upon finding a range of stimulus intervals where complete mechanical fusion exists and where there is a plateau in the heat production. At 0 degrees C, and when muscles are at normal body length, activation heat represents about 40 percent of the heat in an isometric twitch.
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Gibbs et al. (1965) studied this question.
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