Localization of acid phosphatasic activity, phosphate ions and inorganic cations in plant nuclear coiled bodies

In an attempt to clarify the physiology of plant nuclear coiled bodies, three different cytochemical tests related to nuclear RNA metabolism were made: 1)localization of an acid phosphatasic activity; 2) presence of orthophosphate ions; and 3)presence of divalent cations. The three cytochemical tests gave positive results, which are discussed in view of the present knowledge on nuclear RNA synthesis and metabolism.