Key result
Prunus yedoensis bark fractions suppress chemokine induction and STAT1 phosphorylation in keratinocytes.
Answers are grounded in this paper and cite their sources.
Atopic dermatitis (AD) is an inflammatory skin disease commonly characterized by infiltration of inflammatory cells into skin lesions. Keratinocytes produce many chemokines that are involved in the pathogenesis of skin disorders. In particular, macrophage-derived chemokine (MDC/CCL22) and thymus and activationregulated chemokine (TARC/CCL17) are Th2-type cytokines. Serum MDC and TARC levels are increased in AD patients. In this study, we investigated the anti-inflammatory effect and mechanism of action of the active fraction from Prunus yedoensis bark. We evaluated their inhibitory effects on the AD-like inflammatory markers (MDC and TARC) and JAK-STAT pathway (STAT1) in HaCaT keratinocytes. The EtOAc fraction of the crude extract (80% EtOH) and the E5 sub-fraction potently inhibited the induction of MDC and TARC mRNA and protein at 50 <TEX>μg</TEX>/mL in HaCaT cells. In addition, the E5 sub-fraction inhibited the phosphorylation of STAT1 protein associated with IFN-<TEX>γ</TEX> signaling transduction in a dose-dependent manner. Thus, P. yedoensis may have antiatopic activity by suppressing the inflammatory chemokines (MDC and TARC).
No takes yet. Share an insight, caveat, or question.
Kang et al. (2008) studied Atopic dermatitis (in vitro model using IFN-γ-stimulated HaCaT human keratinocytes). Prunus yedoensis bark active fraction (EtOAc fraction of 80% EtOH crude extract and E5 sub-fraction) was evaluated on Inhibition of MDC and TARC mRNA and protein induction and STAT1 phosphorylation. Prunus yedoensis bark fractions (EtOAc and E5) at 50 µg/mL potently inhibited MDC and TARC induction and dose-dependently suppressed STAT1 phosphorylation in HaCaT keratinocytes.
Synapse has enriched 10 related papers. Consider them for comparative context: