Role of CYP3A in Oral Contraceptives Clearance

We evaluated the relative contribution of CYP3A in the overall clearance of commonly used combined oral contraceptives (COCs) based on the results of clinical DDI studies in the literature and new drug applications (NDAs). The results revealed a limited role of CYP3A4 in the metabolism of COC components. Characterization of inhibition or induction spectrum of perpetrators on non-CYP3A pathways might also be crucial in predicting drug interaction potential of an investigational new drug with COCs. COC is the most commonly used contraceptive method in the United States.1 Approximately 9.7 million women at reproductive age are COC users.1 COCs usually contain two synthetic steroid hormones, an estrogen, typically ethinyl estradiol (EE), and a progestin. The commonly used progestins include norethindrone (NET), levonorgestrel (LNG), drospirenone (DRSP), norgestimate (NGM), desogestrel, and gestodene.2 Potential drug-drug interactions (DDIs) should be considered when a medication or an herbal supplement is taken with a hormonal contraceptive (HC). The decreased or increased concentrations of estrogen and progestins due to concomitant medications may lead to unintended pregnancy (loss of efficacy) or increased incidence of adverse events (e.g., increased risk of venous thromboembolism, a rare but severe adverse event). Given the high prevalence of COC use in women and possible consequences of unwanted pregnancy, clinical studies to evaluate the DDI potential between an investigational new drug (that is intended to be used in women with childbearing potential) and COCs have been conducted routinely during the drug development stage and sometimes after drug approval. The metabolic pathways of progestins and EE are not completely understood, as many of these steroids were developed >50 years ago with a limited number of studies. The general consensus is that CYP3A is the major enzyme for oxidative metabolism of EE and the commonly used progestins, including NET, LNG, NGM, and DRSP.3-5 Therefore, most DDI studies assessing the effect of other drugs on the exposure of COCs have been conducted based on the possible interaction via CYP3A. However, there is a certain level of variation among progestins in terms of chemical structures, metabolic pathways, and pharmacokinetic (PK) characteristics. In addition, the significance of CYP3A in the overall disposition of these hormones remains unclear. The objective of this study was to assess the relative contribution of CYP3A in the metabolism of steroid hormone components of COCs using publically available clinical DDI study results. The results from the current assessment might give a new insight on the significance of CYP3A-mediated drug interactions with COCs. The results of DDI studies with COCs were collected via (i) literature search using the electronic databases MEDLINE and PubMed from 1996 to November 2014; (ii) publically available US Food and Drug Administration (FDA) review for new drug applications (Drugs@FDA, http://www.accessdata.fda.gov/scripts/cder/daf/) from 1996 to November 2014; and (iii) the University of Washington Metabolism and Transport Drug Interaction Database (http://www.druginteractioninfo.org).6 The following search terms were applied: drug interactions, contraception and oral contraceptives, EE, DRSP, LNG, NET, and NGM. Four progestins (DRSP, NET, LNG, and NGM) containing COCs were selected for this survey, as they are the most commonly used COCs in the United States and are frequently studied in COC DDI studies.2 The following major criteria were applied for the selection of DDI studies for further analyses: (i) prospective clinical studies conducted in healthy subjects or patients to assess a DDI potential with COCs; and (ii) there is sufficient treatment duration of perpetrators for their inhibitory or induction effect on CYP3A (e.g., inhibitors: treatment duration to achieve steady state or shorter duration if it is consistent with clinical use; inducers: longer than 1 week). In addition, to have a relatively clean data set for further analyses, we excluded some DDI studies based on the following criteria: (i) DDI studies that were conducted based on the likelihood of coadministration without a clear mechanism of drug interactions; (ii) case reports; (iii) perpetrators that have mixed CYP3A4 DDI potential (i.e., both inhibition and induction of CYP3A4); (iv) combination drug products containing multiple CYP3A4 perpetrators, which potentially would result in combined DDI effects (i.e., for two-drug combination, inhibition plus inhibition, induction plus induction, and inhibition plus induction); and (v) perpetrators that did not show inhibition or induction effect on sensitive CYP3A substrates in clinical DDI studies. The sample size of the study and its statistical power were not considered for the selection because there were a limited number of studies that provided its rationale based on the power or sensitivity. If there were multiple studies evaluating the same perpetrators, each of them was included in the final analysis to support the conclusion from each other unless it is/they are not representative of clinical scenarios. If differences were found in the DDI study results, causes would be explored. Based on the selection criteria listed above, selected studies were grouped based on the inhibitory and induction potency of perpetrators on CYP3A (i.e., perpetrators are classified as strong, moderate, or weak inhibitors or inducers of CYP3A based on the criteria described in the FDA's draft DDI guidance).7, 8 Specifically, drugs or herbal supplements that increase the area under the curve (AUC) of a sensitive index CYP3A substrate by greater than or equal to fivefold are considered as strong CYP3A inhibitors. Drugs or herbal supplements that increase AUC of a sensitive index CYP3A substrate by twofold to fivefold or less than twofold are classified as moderate and weak CYP3A inhibitors, respectively. Similarly, strong, moderate, and weak CYP3A inducers should decrease AUC of a sensitive index CYP3A substrate by ≥80%, 50–80%, and 20–50%, respectively. The geometric mean ratio (GMR) of AUC for COCs with and without perpetrators are presented using forest plots to illustrate the effect of CYP3A inhibitors and CYP3A inducers on oral contraceptive exposures (Figures 2-5). The plot illustrates the fold-change and 90% confidence intervals (CIs) for AUC of COCs with and without perpetrators observed in the clinical DDI studies. The shaded area shows the GMR between 0.80 and 1.25, the default no-effect boundary, as specified in the DDI guidance. The DDI data for peak concentration (Cmax) are not discussed in the current assessment because the trend of changes in Cmax are, in general, either similar to or lesser than that of AUC for the DDI studies included in the current assessment. In addition, the underlying mechanisms for the changes in Cmax are more complicated to interpret toward the impact on the systemic clearance than that for AUC. In total, 141 clinical drug interaction studies for these four COC products (EE + NET, EE + LNG, EE + NGM, and EE + DRSP) were collected, among which some were conducted with the same perpetrator-COC drug pair. Of these studies, 33 studies were conducted only based on the likelihood of coadministration without any in vitro or in vivo data to specify underlying mechanisms of interaction. Therefore, they were not included in the data analysis. Six studies were excluded for one or more of the following reasons: (i) they were case reports; (ii) they were perpetrators that are thought to have a mixed effect (both induction and inhibition) on enzymes involved in the metabolism of EE and progestins (e.g., aprepitant and ritonavir (inhibitor and inducer of CYP3A))9-12; and (iii) they were DDI studies with combination drug products, such as Stribild (elvitegravir, cobicistat, emtricitabine, and tenofovir disoproxil fumarate). In addition, 72 studies were excluded from the current review because the perpetrators of these studies had no inhibition or induction effect on sensitive CYP3A substrates, as demonstrated in clinical DDI studies. After reviewing all the studies, 30 studies were selected for further analysis (Figure 1). The magnitude of increase in the exposure (AUC) of EE was not large in the presence of CYP3A inhibitors (Figure 2 and Table 113-26). Strong CYP3A inhibitors, such as voriconazole and ketoconazole, increased EE exposure by 60% and 40%, respectively.13, 14 Telithromycin did not affect the exposure of EE.19 Interestingly, two protease inhibitors, telaprevir and boceprevir, which are known strong CYP3A inhibitors, seemed to decrease the AUC of EE by 26% and 28%, respectively.16, 17 Moderate CYP3A inhibitors, including fluconazole, atazanavir, and faldaprevir, resulted in a 38% to 48% increase in the AUC of EE.20-22, 27 No significant effect on the exposure of EE was observed with other moderate (netupitant23) or weak CYP3A inhibitors.24-26 The effects of CYP3A inhibitors on the systemic exposure of progestins are presented in Figure 3 and Table 1.13-26 Strong CYP3A inhibitors, voriconazole and indinavir, increased the AUC of NET by 53% and 26%, respectively, whereas two strong CYP3A inhibitors, telaprevir and boceprevir, did not significantly affect the exposure of NET.13, 15-17 Coadministration of telithromycin, increased the AUC of LNG by 50%.28 The exposure of DRSP was increased by 100% and 170% when coadministered with boceprevir and ketoconazole, respectively.14, 17, 18, 29 Moderate CYP3A inhibitors showed a modest impact on the exposure of LNG and NET, as demonstrated by <50% increase in the AUCs. Fluconazole, netupitant, and faldaprevir, moderate CYP3A inhibitors, increased the AUC of LNG by ∼25% to up to 40%.21, 23, 27 Unexpectedly, atazanavir, a moderate CYP3A inhibitor, increased the AUC of NET by 110%.22 Weak CYP3A inhibitors seemed to have a negligible effect on the exposure of NET, LNG, and DRSP.24-26 Strong CYP3A inducers, including rifampin, carbamazepine, and phenytoin, reduced the exposure of EE by 38–66% (Figure 4 and Table 230-45). Bosentan and eslicarbazepine, moderate CYP3A inducers, decreased the AUC of EE by 31% and 42%, respectively.30, 31 Efavirenz (a moderate CYP3A inducer) had only 10% reduction in EE exposure, as opposed to a marked effect on progestins, LNG and NGM (Figure 5).32, 33 Oxcarbazepine, which is known as a weak CYP3A inducer, decreased EE exposure by 47%34, 35; nevirapine, a weak inducer, decreased EE exposure by 27%46; perampanel, also a weak inducer, at 4–12 mg daily treatment, did not significantly affect the AUC of EE.36 St John's wort extract, a dietary supplement that exhibited moderate to strong induction on CYP3A4, decreased EE exposure by 14–32%.37, 38 As shown in Figure 5 and Table 2,30-45 strong CYP3A inducers, rifampin and carbamazepine, decreased the AUC of NET by 50–60%.39-41 Carbamazepine and phenytoin decreased the AUC of LNG by 44% and 42%, respectively.42 Moderate CYP3A inducers, efavirenz and eslicarbazepine, decreased LNG exposure by 58% and 37%, respectively.30, 32 Efavirenz also significantly reduced the exposure of norelgestromin (NGMN), a major active metabolite of NGM, by 64%.33 Bosentan, a moderate CYP3A inducer, did not show a significant effect on the exposure of NET.31 The effect of weak CYP3A inducers on progestins varied. Two studies demonstrated that oxcarbazepine significantly decreased the exposure of LNG by 47% and 36%, respectively. Rifabutin and nevirapine showed a small reduction in the AUC of NET (∼10–20%).40, 46, 47 Perampanel demonstrated a dose-dependent effect on the change of the exposure of LNG.48 At the dose of 12 mg per day for 21 days, perampanel decreased the AUC of LNG by 40%, whereas the doses at 8 mg and 4 mg per day had no significant impact on LNG exposure. Lersivirine, another weak CYP3A inducer, slightly decreased the AUC of LNG.43 Two studies demonstrated that St John's wort extract resulted in a 12% reduction on the exposure of NET.37, 38 The current assessments showed that EE and progestins, such as NET and LNG, are minimally sensitive to CYP3A inhibition. The AUC changes of EE and progestins in the presence of a strong CYP3A inhibitor were less than twofold, except for DRSP. Mild inhibitory effect of CYP3A inhibitors on the PK of EE and progestins suggests that CYP3A-mediated oxidation may have limited contribution to the overall disposition of these steroid hormones. EE is extensively metabolized, primarily through intestinal sulfation and hepatic oxidation, glucuronidation and sulfation.3 The oxidative metabolism accounts for the elimination of 30% of EE dose and is catalyzed mainly by CYP3A (67%) and to a minor extent by CYP2C9 (23%).49-51 In addition to CYP-mediated biotransformation, other alternate elimination pathways are glucuronidation by UDP-glucuronosyltransferase 1A1 (UGT1A1) and sulfation by sulfotransferase 1E1 (SULT1E1; Table 35, 14, 17, 29, 49-58). Strong CYP3A inhibitors resulted in no (<1.25-fold) or small increases (<twofold) in EE exposure, suggesting that CYP3A does not contribute significantly to the elimination of EE. Interestingly, two strong CYP3A inhibitors, boceprevir and telaprevir, decreased the exposure of EE. The mechanisms contributing to the decreased exposure of EE remain unclear. The in vitro data indicated that boceprevir and telaprevir have very low or no induction potential for CYP3A.16, 18, 59 Therefore, the reduced EE concentrations are unlikely to be explained by CYP3A induction from boceprevir or telaprevir. The terminal half-life of EE was not changed much when boceprevir was coadministered (14 h and 15 h for EE with and without boceprevir, respectively).17 Based on this observation, it seems that the drug interaction with boceprevir mainly affects the intestine metabolism/efflux (fg) of EE. The EE is a highly permeable drug and its cellular uptake is primarily driven by passive diffusion.3 Although uptake transporters seem to play a minimal role in EE cellular transport, it is possible that EE interacts with efflux transporters, as indicated by the Caco-2 results. In particular, in vitro data showed that EE is a substrate of P-glycoprotein (P-gp), breast cancer resistance protein (BCRP), and multidrug resistance-associated protein (MRP)-2.3 Boceprevir has been reported as an in vitro P-gp inhibitor with limited in vivo inhibition on digoxin clearance.18 Nonetheless, transporter-mediated drug interactions still cannot explain the findings from the DDI study with boceprevir, as reduced intestine efflux of EE arising from boceprevir-mediated inhibition of P-gp would be anticipated to increase EE exposure, rather than decrease EE exposure. The effect of moderate CYP3A inhibitors on the exposure of EE varied. Interestingly, the effect of atazanavir, a moderate CYP3A inhibitor, as specified by the FDA's draft DDI guidance, on the exposure of EE seemed to be unexpectedly stronger than that of strong CYP3A inhibitors except for voriconazole. It has been reported that the glucuronidation clearance pathway is around 35% of the total clearance of EE and EE 3-O-glucuronidation via UGT1A1 is considered to be the major hepatic UGT pathway.3, 60 Considering atazanavir is known to be a UGT1A1 inhibitor,8, 22 the increase in EE exposure in the presence of atazanavir may be partially due to inhibition of UGT1A1. Metabolic characteristics of progestins have not been well addressed. It was proposed that NET and LNG undergo extensive reduction of α, β-unsaturated ketone of ring A in their steroidal structures forming reduced, and, to a lesser extent, hydroxylated metabolites. The parent drugs and their metabolites can be conjugated, forming sulfated and glucuronidated products, which are excreted primarily in urine, and also in feces5, 61 (Table 35, 14, 17, 29, 49-58). In vitro studies showed that NET is a substrate of CYP3A and the oxidation of NET was inhibited in the presence of ketoconazole, a strong inhibitor of CYP3A.4 Although there is no dedicated study suggesting whether CYP-mediated biotransformation reactions may be of minor relevance compared with reduction and conjugation, the data from current assessment seem to indicate that CYP3A does not play a major role in NET metabolism. In particular, strong CYP3A inhibitors exhibited mild (<50% increase) or no impact on NET exposure. The greatest increase in the exposure of NET was observed (twofold increase) when concomitantly treated with atazanavir, a moderate CYP3A inhibitor. In addition to CYP3A inhibition, atazanavir-mediated inhibitory effect on UGT1A1 may contribute to this interaction.62-64 Thus, observation of more pronounced effects of atazanavir on the systemic exposure of NET may be explained by dual inhibition of CYP3A and UGT1A1. Interestingly, boceprevir and telaprevir also slightly decreased NET exposure, even though they exert a strong inhibitory effect on the metabolism of other CYP3A substrates, such as midazolam. The variable and inconsistent inhibition effect of strong and moderate CYP3A inhibitors on NET may suggest that CYP3A-mediated metabolism is unlikely to be the only major pathway in the clearance of NET and other pathways are likely to also be involved. Mild impact of telithromycin, a strong CYP3A inhibitor, on the exposure of LNG also indicates a limited contribution of CYP3A on the metabolism of LNG. This is consistent with the findings from in vitro studies, in which CYP-medicated biotransformation seemed to be less important compared with reduction and conjugation.65 An in vitro study showed that the biotransformation of DRSP is mainly mediated by reductases, sulfotransferases,29 and CYP 3A contributes only to a minor extent to the metabolism of DRSP (<10%).14, 29 When considering this metabolic fate of DRSP, it is anticipated that an inhibitory effect on CYP3A would have little influence on the exposure of DRSP. However, the multiple dosing of a strong CYP3A inhibitor, ketoconazole, increased the AUC of DRSP by 2.7-fold. The half-life of DRSP was also prolonged when ketoconazole was coadministered.14 Another strong CYP3A inhibitor, boceprevir, increased DRSP exposure by twofold. In vitro data showed that boceprevir is a reversible time-dependent inhibitor of CYP3A, but not an inhibitor of other major CYPs, or of UGT1A1 and UGT2B7.66, 67 In addition, there has been no clinical evidence that ketoconazole or boceprevir inhibit other metabolic enzymes of DRSP, including reductases and based on literature Therefore, to the in vitro the results of clinical DDI studies with strong CYP3A4 inhibitors ketoconazole and boceprevir indicated the contribution of CYP3A to the metabolism of DRSP. Interestingly, in a similar DDI study concomitant dosing of boceprevir showed no impact on NET exposure. with NET and LNG, the significant and consistent increase in the systemic exposure of DRSP by strong CYP3A inhibitors may that CYP3A a more important role in the metabolism of DRSP than that of NET and LNG. Although the impact of CYP3A inhibition on the metabolism of EE, LNG, and NET is the current demonstrated that the exposure of LNG, NET, and EE was significantly by concomitant dosing of CYP3A In particular, strong CYP3A inducers, such as carbamazepine, rifampin, and phenytoin, and moderate inducer, to a reduction in the exposure of these COC components. It is that these CYP3A inducers have the induction potential of multiple drug including and and enzymes by the the In addition, and can the of some which may play an important role in the biotransformation of NET, LNG, and Therefore, significant decrease in the exposure of EE and progestins in the presence of these inducers is likely to induction on multiple enzymes of steroid hormones including CYP3A, and St John's wort has been known to be a moderate to strong inducer of enzymes such as It was reported that of St John's wort may lead to a contraception The AUC decrease following with St John's wort for both NET and EE in two studies was no more than 38 suggesting a minor It is that the induction effect of John's wort on CYP enzymes and be The current may have in the results. The DDI findings were collected from studies, which had study and treatment of and The dose and dosing dose multiple of perpetrators may play an important role in the magnitude of induction or inhibition In addition, some studies included in this might have for such as sample size and treatment this study did not the inhibition or induction potential on drug transporters that may also be involved in the disposition of EE or certain in vitro data showed that efflux transporters, including and play a role in EE efflux and EE (a major metabolite of is a substrate of and However, there have been no dedicated studies the impact of these transporters on the overall disposition of EE and This assessment showed that coadministration of strong and moderate CYP3A inhibitors to a modest increase in the systemic exposure of EE, LNG, and NET with the of DRSP, suggesting that the contribution of CYP3A in the metabolism of EE, NET, and LNG is not DRSP seemed to be more sensitive to CYP3A inhibition compared with NET and LNG. In strong CYP3A inducers seemed to a marked reduction in the systemic exposure of EE, LNG, NET, and NGM It may be explained by the that CYP3A inducers used for studies had induction potential of multiple drug enzymes including and in addition to enzymes transporters involved in the disposition of EE and of inhibition or induction spectrum of perpetrators on metabolic pathways (e.g., glucuronidation and transporters should be considered in predicting and the overall DDI potential with COCs. As observed from this survey, the same had effects on progestins combination with which a in the study findings of one containing hormonal contraception to the other The results may also a on the selection of progestins for DDI assessment based on characteristics. is to the metabolic and pathways of EE and progestins as well as relative contribution of each pathway in their to based PK to the DDI potential with and of The and for their to the current This was by the US Food and Drug of was by an to the at the for Drug and by the for and through an between the US of and the of the study was presented at The no of The described are of the and not the of the US Food and Drug Administration or the US