Disinfection by Ozone Microbubbles Can Cause Morphological Change of Fusarium oxysporum f. sp. melonis Spores

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To investigate the difference in the disinfectant efficiency of ozone microbubbles (O 3 MB) and ozone millibubbles (O 3 MMB), the morphological change of the treated Fusarium oxysporum f. sp. melonis spores was observed with scanning and transmission electron microscopies (SEM and TEM). The disinfectant efficiency of O 3 MB on F. oxysporum f. sp. melonis spores was greater than that of O 3 MMB. On observation with SEM, it was revealed that morphological change of F. oxysporum f. sp. melonis spores was caused by O 3 MB and O 3 MMB, and damage to the spore surfaces by O 3 MB occurred sooner than that by O 3 MMB. On observation with TEM, it was furthermore confirmed that F. oxysporum f. sp. melonis spores treated with O 3 MB induced wavy deformation of cell membrane and the intracellular change different from that with O 3 MMB. Therefore, the greater disinfection efficiency of O 3 MB was suggested to be caused due to the function of the MB in addition to the oxidative power of O 3 .