Ascitic fluid containing high titers of antibody was induced in mice by Staphylococcus. Two to three intraperitoneal inoculations of 0.3 ml of a killed suspension of S. aureus mixed with complete Freund9s adjuvant, given at 5-day intervals, produced ascites in 100 percent of the mice tested.
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Lieberman et al. (1959) studied this question.
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