Emergence of a novel PRRSV-1 strain in mainland China: A recombinant strain derived from the two commercial modified live viruses Amervac and DV

Figures

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  Figure 1. Phylogenetic analysis based on the TZJ2134-L12 and TZJ2134-(A+B) gene sequences and positions of TZJ2134-L12 and TZJ2134-(A+B) in the whole genome structure. (A) Phylogenetic tree based on TZJ2134-L12 of 60 PRRSV-1 strains. The TZJ2134 and DV strains belong to DV-like isolates, which are shown in yellow. (B) Phylogenetic tree based on TZJ2134-(A+B) of 65 PRRSV-1 strains. A novel subgroup DV+Amervac-like isolate is shown in green. (C) The whole genome structure of PRRSV, which shows the positions of 10 open reading frames. TZJ2134-L12 is located at nt 1672-nt 2112 in partial Nsp2, shown in blue; TZJ2134-(A+B) is located at nt 7463-nt 11272 in partial Nsp9, complete Nsp10 and partial Nsp11, shown in yellow. TZJ2134 is indicated with a red triangle in phylogenetic trees.

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  Figure 2. Recombination analysis of TZJ2134-(A+B) and phylogenetic analysis of TZJ2134-A and TZJ2134-B. (A) Recombination analysis of TZJ2134-(A+B). Comparison was performed using TZJ2134-(A+B) as the query sequence and DV (blue) and Amervac (green) as the parent strains. The background color of the DV parental region is gray, whereas that of the Amervac parental region is white. Below the similarity plots is the partial genome structure of PRRSV, which shows the positions of three nonstructural proteins (Nsp9, Nsp10, and Nsp11). (B) Phylogenetic analysis of TZJ2134-A. The parental group (DV-like isolates) is shown in yellow. (C) Phylogenetic analysis of TZJ2134-B. The parental group (Amervac-like isolates) is shown in purple, and the query strain TZJ2134 is indicated with a red triangle.