A glycosidic fraction was extracted from fresh cherry leaves and treated with commercial β-glucosidase and the acetone powder from cherry leaves. After enzymatic hydrolysis, the formation of moderately antifungal benzaldehyde, benzyl alcohol, 2-phenylethanol and coumarin was confirmed by GLC and GC-MS. Thus, it was expected that the precursors of antifungal substances existed as glycosides in the leaves and would be hydrolyzed by the endogenous hydrolytic enzymes when the leaves were damaged. A survey of the constituents in the glycosidic fraction revealed the presence of benzyl p-D-glucoside and 2-phenylethyl β-D-glucoside, and of mandelonitrile β-D-glucosides, sambunigrin and prunasin.
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Ito et al. (1995) studied this question.