Culture-based analysis of oral dysbiosis in post-oncological patients rehabilitated with obturator prostheses

Background Post-oncological maxillary defects are commonly rehabilitated with obturator prostheses. This study compared the oral microbiota of post-oncological patients using obturators with that of control subjects wearing conventional removable dentures, focusing on clinically relevant, cultivable microorganisms.Methods Post-oncological obturator users and denture-wearing controls were examined. Swabs from the oral mucosa, prosthesis surfaces, and resection sites were analyzed using culture-based microbiological methods, including species identification and antimicrobial susceptibility testing. Microbial, demographic, and clinical data were statistically analyzed.Results Obturator users harbored a more pathogenic and antimicrobial-resistant oral microbiota than controls, with increased colonization by opportunistic pathogens such as Staphylococcus aureus (including MRSA), Gram-negative rods (Klebsiella pneumoniae), and yeast-like fungi. S. aureus remained significantly more prevalent in obturator users regardless of radiotherapy status. Candida spp. were prevalent in both groups, with Candida albicans predominating and Candida tropicalis more frequent among obturator users.Conclusions Culture-based analysis demonstrated increased colonization with antimicrobial-resistant opportunistic pathogens in post-oncological patients using obturator prostheses, highlighting the need for intensified hygiene and preventive care.