Key result
IFN-alpha promotes while IFN-gamma inhibits endothelial tube formation, amplified by 12-hour pretreatment.
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The formation of blood vessels in vivo (angiogenesis) is an important process and is usually initiated in response to injury, tumor growth, or normal tissue development. We have studied the effect of human interferon (IFN) alpha (alpha) and gamma (gamma) on the capillary-like network formation in an in vitro model of angiogenesis using human umbilical vein endothelial cells (HUVEC). When HUVEC cells are plated on Matrigel (reconstituted basement membrane matrix enriched in laminin), a network of capillary like structures (endotubes) rapidly forms. IFN-alpha enhanced the tube formation in a dose-dependent manner, whereas IFN-gamma significantly inhibited the tube formation. In addition, both the enhancement and inhibition of angiogenesis by IFN-alpha and gamma was found to be greater if the cells were pretreated with IFN for 12 hr before plating on the Matrigel. These results suggest that IFN may play an important role in several vascular processes including early stages of wound healing, recanalization of thrombi, tumor growth, metastasis, normal growth, and development.
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Maheshwari et al. (1991) studied Angiogenesis (in vitro model using HUVEC). Human interferon alpha (IFN-alpha) and interferon gamma (IFN-gamma) was evaluated on Capillary-like network (endotube) formation on Matrigel. IFN-alpha enhanced and IFN-gamma inhibited capillary-like tube formation in HUVEC on Matrigel, with greater effects seen after 12 hr of IFN pretreatment.
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