In vitro Culture Development and Secondary Metabolites of Dillenia indica Tree

Background and Objective: There are some factors that affected the in vitro behavior of Dillenia indica and secondary metabolites such as different types of culture media, concentrations of BA and NAA as well as the physical state. The aim of this experiment was to study various factors on behaviors of Dillenia indica to attain suitable micropropagation protocol in vitro and in vivo stages. Materials and Methods: The experimental study was carried on Dillenia indica plant to evaluate the effect of three types of different media (MS, WPM and B5) with BA (0.5, 1.0 and 2.0 mg LG 1 ), three physical states (Solid, semi-Solid and liquid) of MS medium with NAA (0.0, 0.2, 0.4 and 0.6 mg LG 1 ) on various morphological and secondary metabolites characters. Results: The maximum numbers of the formed shootlets/explants and leaves/shootlet were recorded for MS medium or WPM+2 mg LG 1 of BA while, B5 medium led to reduce those characters. Low concentration of BA (0.5 mg LG 1 ) supplemented to WPM or B5 medium had stimulation effect on shootlets elongation. The maximum phenol, tannin and pigments contents were found in shootlets that were cultured on WPM+1.0 mg LG 1 BA.