We describe an instrument that extends the state of the art in a single-molecule tracking technology, allowing extended observations of single fluorophores and fluorescently labeled proteins as they undergo directed and diffusive transport in three dimensions. We demonstrate three-dimensional tracking of individual quantum dots undergoing diffusion for durations of over a second at velocities comparable to those of intracellular signaling processes.
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Lessard et al. (2007) studied this question.
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