An assay using the L-929 mouse fibroblast cell culture line was described to evaluate the toxicity of Arlacel A. The toxic lots of AA produced a characteristic cytotoxic effect consisting of intracytoplasmic refractile bodies. The results obtained by the cell culture and mouse assays were similar for 18 of 19 lots tested; one lot was moderately toxic by the cell culture assay only. A correlation was seen between the amount of FFA and the toxicity of AA. The cell culture assay provides a sensitive and reproducible assay to evaluate AA toxicity.
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Barile et al. (1970) studied this question.