A Histone Deacetylase Capable of Deacetylating Chromatin-bound Histone¹

A previous study has shown that histone deacetylase extracted from calf thymus with 0.05 M phosphate buffer, pH 7.0, can deacetylate only free histone and cannot deacetylate chromatin-bound histone. An enzyme capable of deacetylating chromatin-bound histone has now been extracted from calf thymus nuclei with 0.2 M phosphate buffer, pH 7.0. The enzyme differs from the previously known enzyme in chromatographic behavior.